| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062096 |
| Chromosome: | chromosome 16 |
| Location: | 6084079 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676000 | (1 of 102) PF01753 - MYND finger (zf-MYND) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCGGCAGCATAGGCCAGACGCAGCGGT |
| Internal bar code: | GGTTCCGAAACCGTTACGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 95.8042 |
| LEAP-Seq n confirming: | 274 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTATGGACAGGGCAGAC |
| Suggested primer 2: | TGATGCTGCTGAAACCTCTG |