| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062098 |
| Chromosome: | chromosome 10 |
| Location: | 2476292 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436053 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTGAGTCCGCGTGGGGCACCGTTGTCT |
| Internal bar code: | GGCAGGAGGGCGGCCCCGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1063 |
| LEAP-Seq percent confirming: | 87.8481 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTGGGTTCAAAATCAGT |
| Suggested primer 2: | GACCGAAGCAAGGTCTGAAG |