Insertion junction: LMJ.RY0402.062113_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.062113
Chromosome:chromosome 13
Location:1628798
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g573550 ANK26 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGCCATAATCGCCAGTCGCCATCCTAAT
Internal bar code:GCTGGGGACTCATTATCGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1102
LEAP-Seq percent confirming:99.3649
LEAP-Seq n confirming:1408
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGAGGTGGTGCAGCTAT
Suggested primer 2:CTGATGCAGGGGTCTCACTT