Insertion junction: LMJ.RY0402.062121_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.062121
Chromosome:chromosome 2
Location:903676
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g079450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCTGGTCAGTGGTCAGGGTGGCGCTCT
Internal bar code:CGGACCCCGGGGTCCATGTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:300
LEAP-Seq percent confirming:99.7723
LEAP-Seq n confirming:1753
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCGACCTATTTAGCTGC
Suggested primer 2:GCCGCTCTGTCTACTTCCAC