| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062163 |
| Chromosome: | chromosome 7 |
| Location: | 4624176 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344200 | (1 of 3) IPR000104//IPR020846 - Antifreeze protein, type I // Major facilitator superfamily domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGAGATAGGCGGCAGTAGTGCTTGG |
| Internal bar code: | GATCCACCGACGGTAGACCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 99.7062 |
| LEAP-Seq n confirming: | 16966 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGGCCTTATGATGGAGG |
| Suggested primer 2: | CAAGAGTGTGTGTGTGCGTG |