Insertion junction: LMJ.RY0402.062166_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.062166
Chromosome:chromosome 3
Location:2556839
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g160350 ANK7 (1 of 1) PF07714//PF12796 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2); Predicted protein with ankyrin repeats 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGATCAGGTGCTGGCTTGGACAGGATTGG
Internal bar code:GGCAATTCATGAAGGTTGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:692
LEAP-Seq percent confirming:99.3501
LEAP-Seq n confirming:7491
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGAGAGTGCGTTTTGTG
Suggested primer 2:TGGGTTGATGACCAGCTGTA