Insertion junction: LMJ.RY0402.062189_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.062189
Chromosome:chromosome 11
Location:3712862
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g482800 STK5,STPK5 (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Serine/threonine protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATCCCACCCGCTCCACCCCTGTCGCAC
Internal bar code:GTGCTGTACGAAATGGGGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:42
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTAAAGTCGCAAGTGGG
Suggested primer 2:TTTCGATCTCAACCTGGGAC