| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062196 |
| Chromosome: | chromosome 17 |
| Location: | 3622774 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726150 | (1 of 1) PTHR23073:SF29 - P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGTGGCTGGATGTGCGATGGGACAGG |
| Internal bar code: | ATACTCCTGTATGATCGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 99.2852 |
| LEAP-Seq n confirming: | 3056 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGATCTGCCACTCTAAA |
| Suggested primer 2: | AAAAATCGGTCGCATCAAAG |