| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062209 |
| Chromosome: | chromosome 3 |
| Location: | 3061371 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164450 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCACGTTAGCAGGGGTGCTTTGACATAC |
| Internal bar code: | CCGGCTTACCTGTTCACTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1092 |
| LEAP-Seq percent confirming: | 99.5292 |
| LEAP-Seq n confirming: | 8457 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGAGTCTGTCTCAAGTC |
| Suggested primer 2: | CAAGGCCGCTACTCCTACTG |