| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062210 |
| Chromosome: | chromosome 5 |
| Location: | 1232197 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245352 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCCCATCCCCGCACATGCGCGCAGGC |
| Internal bar code: | CGACTGGCGCCTGGCGTTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 97.9888 |
| LEAP-Seq n confirming: | 4385 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCCACACCACTCCACAC |
| Suggested primer 2: | CTCGTTACCGCCGTAATTGT |