| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062219 |
| Chromosome: | chromosome 11 |
| Location: | 1064654 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467674 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCACGAATGGCCAGGCCAGCGTGAAT |
| Internal bar code: | TGCGGTGGTCTGCGCCTCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 98.902 |
| LEAP-Seq n confirming: | 16483 |
| LEAP-Seq n nonconfirming: | 183 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCGTCACCCCAGTAGT |
| Suggested primer 2: | TCGGTACTCACCATCAGCAG |