| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062223 |
| Chromosome: | chromosome 5 |
| Location: | 3307126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTAAAGCGGCGCTGCCGTCGCGTACGCC |
| Internal bar code: | GGAGAGGGCCGCGCACCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.407 |
| LEAP-Seq n confirming: | 161418 |
| LEAP-Seq n nonconfirming: | 963 |
| LEAP-Seq n unique pos: | 158 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAATGCACTTGCAAACA |
| Suggested primer 2: | TTGCGGGTTCAGTAATCCTC |