| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062231 |
| Chromosome: | chromosome 17 |
| Location: | 1744013 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709000 | (1 of 1) PTHR11319//PTHR11319:SF35 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTGCCTCTGCCGCTACATTGATAACT |
| Internal bar code: | GAATGATCGGTGGTGCCTCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 99.7699 |
| LEAP-Seq n confirming: | 867 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTACGGGCACGTATTGAT |
| Suggested primer 2: | GGCACACATGACACACATGA |