| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062249 |
| Chromosome: | chromosome 3 |
| Location: | 831641 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146487 | XPO1 | (1 of 1) K14290 - exportin-1 (XPO1, CRM1); Exportin 1 related protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGGCAGGTGCCGGGCTTGGTAGGGTTG |
| Internal bar code: | ATGCGTGCTAGGCCACGAGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1216 |
| LEAP-Seq percent confirming: | 99.6544 |
| LEAP-Seq n confirming: | 14992 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCTAGACCAGCCGATA |
| Suggested primer 2: | TGCTGAGTAAACATCTGGCG |