| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062252 |
| Chromosome: | chromosome 3 |
| Location: | 8203775 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200000 | (1 of 2) PTHR10183//PTHR10183:SF323 - CALPAIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGGGATGTTGGCCAAAGTTAACGGTT |
| Internal bar code: | TGTACGGAGGATGGAGTGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 99.4578 |
| LEAP-Seq n confirming: | 1651 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAGCTTACAGCAGGCCC |
| Suggested primer 2: | CAAAGCTCCGCATCTACTCC |