| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062255 |
| Chromosome: | chromosome 15 |
| Location: | 1073733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638401 | OPR76,RAP12,CLR10 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTAGCGCCCACAGGCTGTTGGATAAT |
| Internal bar code: | GGATGAGTCATTTATGGCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 95.806 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGTCTGCACCAAAACGA |
| Suggested primer 2: | ATCGGTGCATCGTCTTTTTC |