| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062258 |
| Chromosome: | chromosome 12 |
| Location: | 8192548 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550800 | REV1 | (1 of 1) K03515 - DNA repair protein REV1 (REV1); Deoxycytidyl transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCCCCAGTCCTAGTCCCAGTCCCTG |
| Internal bar code: | TGGGGAATGCTTGCCCAATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 99.3781 |
| LEAP-Seq n confirming: | 1598 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTCGTTGACATGCTCTG |
| Suggested primer 2: | GCTGGCAGTAAGAAGCAAGG |