| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062259 |
| Chromosome: | chromosome 9 |
| Location: | 6734996 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409000 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGGGGTGAGGTGGAGGCGCAGGAGCCG |
| Internal bar code: | CCTGTTCTGAATGCCAAGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1374 |
| LEAP-Seq percent confirming: | 99.0678 |
| LEAP-Seq n confirming: | 2338 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTGCTGATGCACTTCCA |
| Suggested primer 2: | ACCTTGTGATGGAGGACCAG |