| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062263 |
| Chromosome: | chromosome 13 |
| Location: | 2812583 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583100 | (1 of 1) 2.4.99.12 - Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase / Lipid IV(A) KDO transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCGCAGTTCTGCATGCAAAGCGTGTT |
| Internal bar code: | CCAGCGACGGCTCCGCAGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.479 |
| LEAP-Seq n confirming: | 20049 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGCAAGACGCAACTAA |
| Suggested primer 2: | GACGTCTAACCTCCGCTCAG |