| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062278 |
| Chromosome: | chromosome_9 |
| Location: | 4739476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g397142 | TRP15 | Transient receptor potential ion channel protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCCAGCCAAAGGGGGTGGGGCTGGGGGTT |
| Internal bar code: | TTCCGACGAACACGCGTAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 30.2235 |
| LEAP-Seq n confirming: | 1082 |
| LEAP-Seq n nonconfirming: | 2498 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACAAGCAGACCGAGA |
| Suggested primer 2: | ACAGTCAATACCGCACCTCC |