| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062281 |
| Chromosome: | chromosome 8 |
| Location: | 1933623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367350 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGCTATCGTTGGACTTGGGCCCGGTC |
| Internal bar code: | TTTCGGGATCAGCTCGCCTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.4165 |
| LEAP-Seq n confirming: | 10733 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACACACTCATATCCCAG |
| Suggested primer 2: | TTGTTGCAGCAGGCAATTAG |