| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062314 |
| Chromosome: | chromosome 6 |
| Location: | 8247829 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306000 | GOX3 | (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase; Glyoxal oxidase 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCTATCAGCTCAAGCACGCCAAGGGC |
| Internal bar code: | GGTTACAAAGCGAACCGGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.6115 |
| LEAP-Seq n confirming: | 2051 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACACCTAGGTCCTTTGGA |
| Suggested primer 2: | GTGAGGCAGTAGCAGGGAAG |