| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062332 |
| Chromosome: | chromosome_4 |
| Location: | 2573876 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g222500 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGCCAACTGGCGGTGCTGGGCGAGCAGCT |
| Internal bar code: | GTGACTACCCGGGCGGCTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 96.5183 |
| LEAP-Seq n confirming: | 1691 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGATGGAAAGGAGCAA |
| Suggested primer 2: | AGCATGCATTTGACCTTTCC |