Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.062349 |
Chromosome: | chromosome 9 |
Location: | 1854168 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g395750 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCAGCGGCATGTACGGCGGAGCGGTG |
Internal bar code: | ATTTGGGTGGCGTGTAATCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 442 |
LEAP-Seq percent confirming: | 99.37 |
LEAP-Seq n confirming: | 2366 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGTTGTGCTTTCTGGGGT |
Suggested primer 2: | CAGGTACCAGGGCTTCATGT |