| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062351 |
| Chromosome: | chromosome 16 |
| Location: | 4234742 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668400 | (1 of 1) PTHR23354//PTHR23354:SF72 - NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED // PROTEIN LMD-3, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGGCGTGCAACGAGTATAATGGTACT |
| Internal bar code: | GAAATCCGCTCGGCGGTTAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1408 |
| LEAP-Seq percent confirming: | 99.1902 |
| LEAP-Seq n confirming: | 15310 |
| LEAP-Seq n nonconfirming: | 125 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCGTCAGTTCTCTTTGT |
| Suggested primer 2: | AGCAGCAGCATGGATTTCTT |