| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062376 |
| Chromosome: | chromosome_16 |
| Location: | 1887390 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g655750 | TEK1 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCAGCTGGCGCCAACCCAATATTCCGCAA |
| Internal bar code: | GGCTATAACGTGTCCGAAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTAGTTTGGGCTGGTA |
| Suggested primer 2: | ATGACAAGTTTACAGGGCCG |