| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062390 |
| Chromosome: | chromosome 12 |
| Location: | 3396765 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496950 | (1 of 1) 3.4.13.5 - Xaa-methyl-His dipeptidase / X-methyl-His dipeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTGTGCGGGTCCCATGCATGCGCCTCG |
| Internal bar code: | GTTTGAGGACCGGACGATTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 99.4194 |
| LEAP-Seq n confirming: | 2911 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGATGGACATGCTGTG |
| Suggested primer 2: | CATAGACGACTTGGGGATGG |