| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062400 |
| Chromosome: | chromosome 12 |
| Location: | 551133 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492851 | EBM7 | (1 of 4) K19355 - mannan endo-1,4-beta-mannosidase (MAN); Mannan endo-1%252C4-beta-mannosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCATGCATATCCCACACATGGCAGCAG |
| Internal bar code: | CGTTTATTTTGCAGTTCTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 88.2006 |
| LEAP-Seq n confirming: | 598 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGGTAGGGAGAGGGA |
| Suggested primer 2: | GCAAAGATGCAGGAGACACA |