| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062422 |
| Chromosome: | chromosome 12 |
| Location: | 9190201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541950 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCACTGTTCACTTTGCAGGACATCGCG |
| Internal bar code: | TGACAGCCCAGGCGAGCAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 95.2744 |
| LEAP-Seq n confirming: | 625 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGAAACTCGAGCACTTAC |
| Suggested primer 2: | CACGCCAAGACCCATTAGTT |