| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062432 |
| Chromosome: | chromosome 9 |
| Location: | 6802333 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409650 | (1 of 18) PF12906 - RING-variant domain (RINGv) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGTTCCGGAACAGAGAGTGTAGTGCAA |
| Internal bar code: | TTCTCCGCTTGGGAGACAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 92.283 |
| LEAP-Seq n confirming: | 861 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCCTTGACCTTGACCTC |
| Suggested primer 2: | TGCACGTTGACGGATATCAT |