Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.062454 |
Chromosome: | chromosome_12 |
Location: | 5290105 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g528850 | DLX1,DLC3 | Flagellar outer dynein arm 16 kDa light chain, LC3 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGGGGGGGCAGGGGCTCACCGTGAGGAAC |
Internal bar code: | TCTGTGAAATAGTCTGGCTAAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 478 |
LEAP-Seq percent confirming: | 99.4641 |
LEAP-Seq n confirming: | 7981 |
LEAP-Seq n nonconfirming: | 43 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGCTTTCGTCTCCTGCAT |
Suggested primer 2: | ATCCTGAGCATACCCCACAG |