Insertion junction: LMJ.RY0402.062457_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.062457
Chromosome:chromosome 13
Location:4737724
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g604850 (1 of 1) IPR026126 - BRISC and BRCA1-A complex member 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGGATTGGAAGGGACCCCCCCCCCCCT
Internal bar code:GGGCAGTCATAGGGGCGGAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:217
LEAP-Seq percent confirming:98.6907
LEAP-Seq n confirming:603
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACATACACCAGCAT
Suggested primer 2:GGAGAGGGTGTGTGTGTGTG