| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062471 |
| Chromosome: | chromosome 8 |
| Location: | 3700303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376800 | MIF2 | Mitochondrial translation initiation factor 2; (1 of 1) PTHR23115//PTHR23115:SF184 - TRANSLATION FACTOR // TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAACAGCGTGAAATGGACTTGGCGTCTT |
| Internal bar code: | ATCACAAATTGTTATCCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 92.323 |
| LEAP-Seq n confirming: | 44568 |
| LEAP-Seq n nonconfirming: | 3706 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTACAAGAGGGACCTG |
| Suggested primer 2: | GGTTTGATTCAGTACCGCGT |