| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062483 |
| Chromosome: | chromosome 9 |
| Location: | 673252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403050 | LSF2,DSP6 | Laforin glucan phosphatase (SEX4)-like 2; (1 of 1) PTHR10159//PTHR10159:SF326 - DUAL SPECIFICITY PROTEIN PHOSPHATASE // LAFORIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATAACAGCTTTAAAATGCAGCCGGCCGG |
| Internal bar code: | GGGTTTGGTTCACGCGTTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.4269 |
| LEAP-Seq n confirming: | 347 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGTCGAACGTCGTGTGT |
| Suggested primer 2: | AGTATTGTGGGGATTGCTGC |