Insertion junction: LMJ.RY0402.062500_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.062500
Chromosome:chromosome 13
Location:1234162
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g570350 AKC4 (1 of 1) PTHR10566//PTHR10566:SF74 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC TRANSPORTER-LIKE; conserved expressed ABC-1 like kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGATTCCCATCCATGCACACACATACACA
Internal bar code:TTTCACGTTTTGGGACTCAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:79.8198
LEAP-Seq n confirming:443
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCCTCGATCTTGTTGGG
Suggested primer 2:AGCATACAAACGGTTGAGGG