Insertion junction: LMJ.RY0402.062515_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.062515
Chromosome:chromosome 8
Location:888083
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g361400 (1 of 3) PF03638 - Tesmin/TSO1-like CXC domain, cysteine-rich domain (TCR) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACGACGACCGAAACAAGACGAGTGCCA
Internal bar code:GTCTCACGTTAGCTCCAATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:99.9083
LEAP-Seq n confirming:7627
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGAGGACGCGATAGACA
Suggested primer 2:TGTGACAGTGAAGTGCACGA