| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062521 |
| Chromosome: | chromosome 16 |
| Location: | 7759800 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692116 | (1 of 3) PTHR23505:SF12 - MAJOR FACILITATOR PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAATAATATGATGACACCTTGAGTTGTG |
| Internal bar code: | ATGTTCGAAGATTAACTCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1114 |
| LEAP-Seq percent confirming: | 99.4443 |
| LEAP-Seq n confirming: | 3579 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTTCGAGACTGAACCC |
| Suggested primer 2: | ACCTTGTGTCTTCAATGCCC |