| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062532 |
| Chromosome: | chromosome 8 |
| Location: | 4641958 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGAACTGAACCCGGCAGCTGAGGTGT |
| Internal bar code: | AAGCTGACTTCGTTGGGAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 89.67 |
| LEAP-Seq n confirming: | 8533 |
| LEAP-Seq n nonconfirming: | 983 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACGTACGTGGATTGCAT |
| Suggested primer 2: | CGACAAAACTGAGCACCAGA |