Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.062533 |
Chromosome: | chromosome 3 |
Location: | 5674911 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g187600 | (1 of 15) PF13365 - Trypsin-like peptidase domain (Trypsin_2) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCCACTACCGTACTCAAGGTGCAAAGC |
Internal bar code: | GCCATACATGAGCCGAGCGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1024 |
LEAP-Seq percent confirming: | 96.7867 |
LEAP-Seq n confirming: | 22018 |
LEAP-Seq n nonconfirming: | 731 |
LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGCTGCAACTGAAGCTGAC |
Suggested primer 2: | CCGTCAGTACGCCATAGGTT |