Insertion junction: LMJ.RY0402.062533_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.062533
Chromosome:chromosome 3
Location:5674911
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g187600 (1 of 15) PF13365 - Trypsin-like peptidase domain (Trypsin_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCCACTACCGTACTCAAGGTGCAAAGC
Internal bar code:GCCATACATGAGCCGAGCGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1024
LEAP-Seq percent confirming:96.7867
LEAP-Seq n confirming:22018
LEAP-Seq n nonconfirming:731
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGCAACTGAAGCTGAC
Suggested primer 2:CCGTCAGTACGCCATAGGTT