Insertion junction: LMJ.RY0402.062539_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.062539
Chromosome:chromosome_9
Location:3256790
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g387912 FAP139 Flagellar Associated Protein, serine endopeptidase sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGCCCTGCCAGCCTCGCATCACCAAGCCTG
Internal bar code:TTTGGCCGCGCGCGCATAGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:361
LEAP-Seq percent confirming:99.777
LEAP-Seq n confirming:1342
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCTAGCCAGGTGCAGA
Suggested primer 2:CTGTGAGCAGCTCGTTCTTG