| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062556 |
| Chromosome: | chromosome 7 |
| Location: | 5895667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353950 | XPG5 | DNA repair 5'-3' exonuclease; (1 of 1) 3.1.11.1 - Exodeoxyribonuclease I / Exonuclease I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGCCGCTCAGCCTCCGCATCCCCGCAC |
| Internal bar code: | GGTCTGCTAATTCTAGGCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 99.6814 |
| LEAP-Seq n confirming: | 2190 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCATCTATGACGGCTTG |
| Suggested primer 2: | TCATCCTCGTCCTCCATTTC |