Insertion junction: LMJ.RY0402.062562_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.062562
Chromosome:chromosome 17
Location:181199
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697406 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATATCATGCGGGCGGTCGACGGGCACAAG
Internal bar code:CCCGGCACACGACAGGGTGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:99.5854
LEAP-Seq n confirming:2162
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCGCAGGATGATGTGAC
Suggested primer 2:GGAGCTCAAGCAGGGTGTAG