| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062564 |
| Chromosome: | chromosome 8 |
| Location: | 4549787 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382800 | CDPK13 | (1 of 1) PF00036//PF07714//PF13499 - EF hand (EF-hand_1) // Protein tyrosine kinase (Pkinase_Tyr) // EF-hand domain pair (EF-hand_7); Calcium/calmodulin-dependent protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATATGCTACAACACACAAAGCACGCTCA |
| Internal bar code: | TTGTAATTCACAAACGAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 99.8431 |
| LEAP-Seq n confirming: | 1273 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTTCTTTGACCTGCTGC |
| Suggested primer 2: | GCCTGCTCAAAGGTTCAGTC |