Insertion junction: LMJ.RY0402.062581_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.062581
Chromosome:chromosome 17
Location:1611300
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g707900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCATGTTTGTCGAGGCACCCCAAGAGTC
Internal bar code:AGGCGCGTGTGGGTGAGTAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:828
LEAP-Seq percent confirming:99.9215
LEAP-Seq n confirming:2547
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAACTTATCGAACGGACG
Suggested primer 2:CTTTCGTTGCCTTCTTTTGC