| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062593 |
| Chromosome: | chromosome 17 |
| Location: | 2731384 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718185 | (1 of 19) PF00534 - Glycosyl transferases group 1 (Glycos_transf_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTACCCTCCCGTTAGACCAGCCGCGCACT |
| Internal bar code: | GCTGACGACGACGTGCGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1379 |
| LEAP-Seq percent confirming: | 99.6373 |
| LEAP-Seq n confirming: | 12635 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCTGCGACTGCATTAAA |
| Suggested primer 2: | GTGATGAGGAGGGTGAGGAA |