| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062595 |
| Chromosome: | chromosome 14 |
| Location: | 1154775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615634 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCCGCCACCTTGGGCGTGATGGTTGCA |
| Internal bar code: | TTTACTGGCACGGACCCAGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 94.4862 |
| LEAP-Seq n confirming: | 377 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTGTCAACATAACGCA |
| Suggested primer 2: | GTCAAAGCTTTTCCTGCGTC |