| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062626 |
| Chromosome: | chromosome 9 |
| Location: | 1285715 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399200 | SDR19 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGGCGCCACGCCGTTGACACGGATGCC |
| Internal bar code: | GGCAGCCTGTGGGGAAAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 85.8148 |
| LEAP-Seq n confirming: | 732 |
| LEAP-Seq n nonconfirming: | 121 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GAGCTGGGACATCTGGACAT |