| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062630 |
| Chromosome: | chromosome 4 |
| Location: | 3946140 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g231222 | CPN60A,CPN60 alpha | Chaperonin 60A; (1 of 1) PTHR11353:SF11 - CHAPERONIN 60 SUBUNIT ALPHA 1, CHLOROPLASTIC | CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGAACCACCTTTATAGCCGCCCAAATG |
| Internal bar code: | TTACTCTAATCTGGCCGCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 99.1521 |
| LEAP-Seq n confirming: | 10642 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACCTCACACAGTCACA |
| Suggested primer 2: | ATGATGCTGGTGAATGTGGA |