| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062655 |
| Chromosome: | chromosome 7 |
| Location: | 1062612 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319650 | FXL4,FXL5 | FixL-like PAS domain protein; (1 of 3) IPR000014//IPR013767 - PAS domain // PAS fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCGTGCGCGGCGGGTGTCACGGGTGCA |
| Internal bar code: | CGCTCAGTGTAGGGTGTTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.7442 |
| LEAP-Seq n confirming: | 5459 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGCCGTGCTAATAGGGTG |
| Suggested primer 2: | GCTGGATGCTCAACTGTGAA |