| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062722 |
| Chromosome: | chromosome 4 |
| Location: | 2814225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224100 | URN2 | Inosine/uridine nucleosidase; (1 of 1) 3.2.2.8 - Ribosylpyrimidine nucleosidase / Pyrimidine nucleosidase | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAAAGAAATAAGCTGTGGGGCATTGAC |
| Internal bar code: | CATATAACATTAGTTGGTACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 99.7957 |
| LEAP-Seq n confirming: | 7817 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTTTATTTGGGGCGAC |
| Suggested primer 2: | TAGGTCGCACTGCATTTCTG |